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GenScript ToxinEraser™ is an endototoxin removal resin of high efficiency. It is based on the affinity matrix of modified polymyxin B (PMB), which allows highly efficient endotoxin removal. The final endotoxin level can be decreased to less than 0.1 EU/ml with repeat use of ToxinEraser™ endotoxin removal resin. Final removal efficiency may vary depending on the sample type/source.

產(chǎn)品概述

Product Description：

Lipopolysaccharide (LPS) is a bacterial endotoxin, and a major constituent of the cell walls of gram- negative bacteria. During gram-negative bacteremia or endotoxemia, LPS is the principal pathophysiological mediator by which bacteria can cause hypotension, organ failure, disseminated intravascular coagulation, and fatal shock in mammalian hosts. Removal of endotoxin from drugs, injectables, and other biological products is necessary but can also be very difficult.
GenScript’s ToxinEraser™ is an endototoxin removal resin of high efficiency. It is based on affinity matrix of modified polymyxin B (PMB), which allows highly efficient endotoxin removal. The final endotoxin level can be decreased to less than 0.1 EU/ml with repeated use of ToxinEraser™ endotoxin removal resin. Final removal efficiency may vary depending on the sample type/source.

Key Features

• High stability and high removal efficiency
  

• High binding capacity: > 2, 000, 000 EU / ml (CV)
  

• Fast flow without any constant speed pump
  

• Reusable up to five times if properly regenerated
  

• Ready-to-use reagents and materials, such as equilibration buffer, collection tubes, etc.

產(chǎn)品簡介

Product Information：Characteristics of ToxinEraser™ Endotoxin Removal Resin

Size：1.5 ml in pre-packed column

Binding Capacity：Up to 2,000,000 EU/ml resin

Ligand：Modified PMB (Polymixin B)

pH Stability：pH 5 -10

Support Matrix：4% cross-linked agarose, spherical beads

Mean Particle Size：90 μm

Storage：2°C to 8°C (Do not freeze.)

Equilibration Buffer：Phosphate-Buffer, pH 8.0

Regeneration Buffer：Sodium Phosphate-Buffer, pH 8.0; Triton-114

Types of substances that can be applied to the column：Purified recombinant proteins, peptides, antibodies, polysaccharides, etc.

Tolerable Ionic Strength：0.1 to 0.5 M NaCl

Tested reagents that do not interfere with performance：20% DMSO, 20% ethanol, 20% glycerol;1 M urea, 300 mM imidazole; 0.05% Tween 20, 10 mM DTT.

Storage

Regeneration and storage of the Column.
To regenerate the column, wash the column with 10 ml of equilibration buffer and allow the column to drain completely. Add ethanol to the regeneration buffer to a final concentration of 20%, adding at least 1.5 ml to the column for storage. Store at 2-8°C. Do not freeze.

Kit Contents

Kit Contents （Cat. No.:L00338）：PK

ToxinEraser™ Endotoxin Removal Resin：1.5 ml pre-packed column

Regeneration Buffer：125 ml

Equilibration Buffer：125 ml

Flow-Speed Control：1

Collection Tubes：1 bag (3 Tubes)

Tips (1 ml)：2 bags (6 Tips/bag)

Protocol：1

For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.