HFL1 人肺成纖維細胞
| 參考價 | ￥ 1 |
| 訂貨量 | ≥1 件 |
- 公司名稱 上海復(fù)祥生物科技有限公司
- 品牌 其他品牌
- 型號 HFL1
- 產(chǎn)地
- 廠商性質(zhì) 生產(chǎn)廠家
- 更新時間 2025/7/19 16:29:13
- 訪問次數(shù) 5271
聯(lián)系方式：李經(jīng)理15000266580 查看聯(lián)系方式
聯(lián)系我們時請說明是化工儀器網(wǎng)上看到的信息，謝謝!
| 供貨周期 | 一周 | 規(guī)格 | T25 |
|---|---|---|---|
| 貨號 | HFL1 | 應(yīng)用領(lǐng)域 | 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥/生物制藥 |
| 主要用途 | 科學研究 |
CCL-153 HFL1 人肺成纖維細胞，原代細胞|細胞系|細胞株|菌種原代細胞、細胞系。細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！
ATCC® Number: CCL-153™
Designations: HFL1
Depositors: RG Crystal
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens （human）
Morphology: fibroblast
CCL-153 HFL1 人肺成纖維細胞
Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Reverse Transcript: negative
DNA Profile （STR）: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 7,9
TPOX: 6,9
vWA: 17
Cytogenetic Analysis: normal human; diploid; stable
Isoenzymes: G6PD, B
Age: fetus
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2004
recommended serum:ATCC 30-2020
References: 23400: Breul SD, et al. Control of collagen production by human diploid lung fibroblasts. J. Biol. Chem. 255: 5250-5260, 1980. PubMed: 7372635
CCL-153 HFL1
采購中心
化工儀器網(wǎng)